Journal: Structure (London, England : 1993)

Article Title: Inhibition of the pneumococcal virulence factor StrH and molecular insights into N-glycan recognition and hydrolysis.

doi: 10.1016/j.str.2011.08.011

Figure Lengend Snippet: Figure 1. The Modular Architecture of StrH from S. pneumoniae (Strain TIGR4) Both catalytic modules are shown in black and the ‘‘unclassified’’ G5 modules in white. The white and gray boxes at the N and C termini, respectively, correspond the secretion signal and the LPXTG cell-wall anchoring motif. Amino acid boundaries of the catalytic domains are indicated above the schematic.

Article Snippet: Gene fragments encoding both GH20A and B modules (GH20A/B, amino acids 181-984) and the individual GH20 catalytic modules (GH20A, residues 181-614; GH20B, residues 627-1039) were amplified by PCR from S. pneumoniae TIGR4 genomic DNA (American Type Culture Collection BAA-334D) using specific primers to introduce a 50 NdeI and 30 XhoI restriction sites (see Table S1 available online for oligonucleotide primer sequences).

Techniques:

Journal: Structure (London, England : 1993)

Article Title: Inhibition of the pneumococcal virulence factor StrH and molecular insights into N-glycan recognition and hydrolysis.

doi: 10.1016/j.str.2011.08.011

Figure Lengend Snippet: Figure 7. Inhibitors of Strh Increase Opsonophagocytic Killing of S. pneumoniae Survival of S. pneumoniae TIGR4 in neutrophil killing assays, showing comparisons of wild-type (filled bars) and Dstrh strain (open bars) in the presence and absence of NGT or PUGNAc. Asterisks above sample bars represent statistical comparison of that sample with the reference, which is the TIGR4 strain with no inhibitor. Statistical differences were analyzed by unpaired Student’s two-tailed t test. Data are mean values compiled from two independent experiments performed in duplicate ± standard error of the mean. *p < 0.05; **p < 0.01; ***p < 0.001. The Dstrh samples with inhibitors were compared with Dstrh in the absence of inhibitors and were found to have p values >0.1 and thus were not significantly different.

Article Snippet: Gene fragments encoding both GH20A and B modules (GH20A/B, amino acids 181-984) and the individual GH20 catalytic modules (GH20A, residues 181-614; GH20B, residues 627-1039) were amplified by PCR from S. pneumoniae TIGR4 genomic DNA (American Type Culture Collection BAA-334D) using specific primers to introduce a 50 NdeI and 30 XhoI restriction sites (see Table S1 available online for oligonucleotide primer sequences).

Techniques: Comparison, Two Tailed Test

Journal: Microorganisms

Article Title: Effect of Fluidized Bed Drying, Matrix Constituents and Structure on the Viability of Probiotic Lactobacillus paracasei ATCC 55544 during Storage at 4 °C, 25 °C and 37 °C

doi: 10.3390/microorganisms10010074

Figure Lengend Snippet: The storage stability of fluidized bed-dried Lactobacillus paracasei powders embedded in whole milk powder matrix with ( a ) a w 0.3, ( d ) a w 0.4, ( g ) a w 0.5; skim milk powder matrix with ( b ) a w 0.3, ( e ) a w 0.4, ( h ) a w 0.5; milk protein isolate matrix with ( c ) a w 0.3, ( f ) a w 0.4, ( i ) a w 0.5. The viability is expressed as the logarithmic values of survival against a storage time of 4 weeks at 4 °C (black circle), 25 °C (white triangle), and 37 °C (black square). Error bars represent the standard deviation of means ( n ≥ 3).

Article Snippet: This biofilm-like structure and the protective effect may possibly be enhanced by the presence of exopolysaccharides and the adhesive properties of L. paracasei ATCC 55544 [ , ].

Techniques: Standard Deviation

Journal: Microorganisms

Article Title: Effect of Fluidized Bed Drying, Matrix Constituents and Structure on the Viability of Probiotic Lactobacillus paracasei ATCC 55544 during Storage at 4 °C, 25 °C and 37 °C

doi: 10.3390/microorganisms10010074

Figure Lengend Snippet: Scanning Electron Microscopy (SEM) images of fluidized bed-dried Lactobacillus paracasei powders in MPI ( a ), SMP ( b ) or WMP ( c ).

Article Snippet: This biofilm-like structure and the protective effect may possibly be enhanced by the presence of exopolysaccharides and the adhesive properties of L. paracasei ATCC 55544 [ , ].

Techniques: Electron Microscopy

Journal: Microorganisms

Article Title: Effect of Fluidized Bed Drying, Matrix Constituents and Structure on the Viability of Probiotic Lactobacillus paracasei ATCC 55544 during Storage at 4 °C, 25 °C and 37 °C

doi: 10.3390/microorganisms10010074

Figure Lengend Snippet: CLSM image of ( a ) surface of the WMP matrix, showing the immobilization of probiotic Lactobacillus paracasei within the fat and protein layers ( b ) cross section of the matrix, ( c ) spatial distribution of live and dead bacteria which are located below the surface, comprising of bacteria embedded in the protein and lactose matrix. Green live bacteria, red dead bacteria. The scale bar represents 20 µm.

Article Snippet: This biofilm-like structure and the protective effect may possibly be enhanced by the presence of exopolysaccharides and the adhesive properties of L. paracasei ATCC 55544 [ , ].

Techniques: Bacteria

Journal: Journal of neuroinflammation

Article Title: Probiotic treatment induces sex-dependent neuroprotection and gut microbiome shifts after traumatic brain injury.

doi: 10.1186/s12974-025-03419-1

Figure Lengend Snippet: Fig. 1 Schematic representation of the experimental design for acute and chronic TBI models with P Pan-Probiotic (PP) treatment. (a) Acute TBI Model: Mice received either water (VH) or a Pan-Probiotic (PP) cocktail containing Lactobacillus plantarum, L. reuteri, L. helveticus, L. fermentum, L. rhamnosus, L. gas seri, and L. casei via drinking water for 7 weeks prior to TBI induction. Acute assessments were performed 3 days post-injury (dpi), including microbiome sequencing, gut pathology analysis, histological examination, and blood collection. (b) Chronic TBI Model: Mice were pretreated with either water (VH) or the PP cocktail for 2 weeks before TBI or sham surgery. Post-TBI, treatment continued for 5 weeks, and outcomes were evaluated at multiple time points (7, 14, 21, and 35 dpi). Rotarod testing (R) was conducted to assess motor function, and behavioral tests, including the Elevated Plus Maze and Forced Swim Test, were performed to evaluate anxiety- and depressive-like behaviors. Additional analyses included microbiome sequencing, gut pathology, blood collection, histology, and RNAscope imaging at 35 dpi

Article Snippet: The included strains were Lactobacillus gasseri (ATCC 33323), Lactobacillus plantarum (ATCC BAA-793), Lactobacillus reuteri (ATCC 23272), Lactobacillus helveticus (ATCC BAA-2840), Lactobacillus fermentum (ATCC 23271), Lactobacillus rhamnosus (ATCC BAA-2836), and Lactobacillus casei (ATCC BAA-2843).

Techniques: Sequencing, RNAscope, Imaging

Journal:

Article Title: Differentiation of Lactobacillus Species by Molecular Typing

doi:

Figure Lengend Snippet: Molecular typing of Lactobacillus isolates with respect to ATCC typed strains

Article Snippet: The nomenclature used here for the type and reference strains was that given by ATCC and NCFB prior to 1993, and that used for the clinical and stock isolates was the same as that reported in publications by our group ( 23 , 24 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Original nomenclature Ribotype with Bcl I Chromotype with Bcl I L. rhamnosus ATCC 7469 T A 1.1 L. rhamnosus ATCC 11443 A 1.1 L. casei subsp. rhamnosus 81 A 1.1 L. casei subsp. rhamnosus 76 A 1.2 L. rhamnosus ATCC 8530 A 1.3 L. casei subsp. rhamnosus GR-1 A 1.4 L. casei subsp. rhamnosus 36 A 1.4 Vaginal isolate w A 1.4 Vaginal isolate g A 1.4 L. rhamnosus NCFB 2964 A 1.5 L. casei subsp. rhamnosus RC-17 A 1.6 L. fermentum A60 A 1.6 L. plantarum RC-6 A 1.7 L. rhamnosus ATCC 21052 A 2.1 C1-A A 2.1 C3-A A 2.1 C3-B A 2.1 C5-A A 2.1 C7-A A 2.1 C7-B A 2.1 L. casei subsp. casei ATCC 393 B 10.1 L. casei subsp. casei ATCC 4940 B1 11.1 L. paracasei subsp. paracasei ATCC 25302 T C 20.1 L. casei subsp. casei ATCC 334 C 20.2 L. casei subsp. casei ATCC 4007 C1 21.1 L. casei subsp. casei ATCC 4913 C2 22.1 L. casei subsp. casei 55 C2 23.1 L. paracasei subsp. paracasei ATCC 25303 C2 24.1 L. casei subsp. rhamnosus 43 C3 25.1 L. casei 8 C3 25.1 L. casei 65 C3 25.1 L. plantarum ATCC 14917 T D 30.1 L. plantarum UH 2153 D 30.1 L. plantarum ATCC 10012 D 30.2 L. plantarum ATCC 11974 D1 31.1 L. plantarum ATCC 14431 D2 32.1 L. plantarum ATCC 8014 D3 33.1 L. plantarum 260 D3 33.1 L. plantarum RC-20 D4 34.1 L. acidophilus 75 D5 34.2 US2-A D6 35.1 US2-B D6 35.1 US2-C D6 35.1 US3-A D7 35.2 US3-B D7 35.2 US6-A D8 36.1 US6-B D8 36.1 US6-C D8 36.1 C4-A D8 36.1 C4-B D8 36.1 US8-A D9 37.1 L. fermentum ATCC 14931 T E 40.1 L. fermentum ATCC 23271 E1 40.2 L. fermentum ATCC 8289 E2 40.3 L. fermentum ATCC 9338 E2 40.3 L. fermentum ATCC 11739 E3 41.1 L. fermentum ATCC 14932 E4 42.1 L. fermentum B-54 E5 43.1 L. acidophilus RC-14 E5 43.1 L. acidophilus RC-14 (subcultured 20 times) E5 43.1 L. acidophilus ATCC 4356 T F 50.1 US4 F 50.2 L. acidophilus T-13 F1 51.1 L. jensenii ATCC 25258 T G 60.1 Open in a separate window Molecular typing of Lactobacillus isolates with respect to ATCC typed strains Nine health food products were examined.

Techniques:

Journal:

Article Title: Differentiation of Lactobacillus Species by Molecular Typing

doi:

Figure Lengend Snippet: Autoradiogram of Southern blot of BclI-digested DNA after hybridization with 32P-labelled rRNA from E. coli. Lanes 1 to 12 represent ribotype groups A to F. Lane 1, L. rhamnosus ATCC 7469T; lane 2, L. casei subsp. casei ATCC 393; lane 3, L. paracasei subsp. paracasei ATCC 25302T; lane 4, L. casei subsp. casei ATCC 4007; lane 5, L. paracasei subsp. paracasei ATCC 25303; lane 6, L. casei 8; lane 7, L. plantarum ATCC 14917T; lane 8, L. plantarum ATCC 11974; lane 9, L. plantarum ATCC 14431; lane 10, L. fermentum B-54; lane 11, L. acidophilus ATCC 4356T; lane 12, L. acidophilus T-13.

Article Snippet: The nomenclature used here for the type and reference strains was that given by ATCC and NCFB prior to 1993, and that used for the clinical and stock isolates was the same as that reported in publications by our group ( 23 , 24 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Original nomenclature Ribotype with Bcl I Chromotype with Bcl I L. rhamnosus ATCC 7469 T A 1.1 L. rhamnosus ATCC 11443 A 1.1 L. casei subsp. rhamnosus 81 A 1.1 L. casei subsp. rhamnosus 76 A 1.2 L. rhamnosus ATCC 8530 A 1.3 L. casei subsp. rhamnosus GR-1 A 1.4 L. casei subsp. rhamnosus 36 A 1.4 Vaginal isolate w A 1.4 Vaginal isolate g A 1.4 L. rhamnosus NCFB 2964 A 1.5 L. casei subsp. rhamnosus RC-17 A 1.6 L. fermentum A60 A 1.6 L. plantarum RC-6 A 1.7 L. rhamnosus ATCC 21052 A 2.1 C1-A A 2.1 C3-A A 2.1 C3-B A 2.1 C5-A A 2.1 C7-A A 2.1 C7-B A 2.1 L. casei subsp. casei ATCC 393 B 10.1 L. casei subsp. casei ATCC 4940 B1 11.1 L. paracasei subsp. paracasei ATCC 25302 T C 20.1 L. casei subsp. casei ATCC 334 C 20.2 L. casei subsp. casei ATCC 4007 C1 21.1 L. casei subsp. casei ATCC 4913 C2 22.1 L. casei subsp. casei 55 C2 23.1 L. paracasei subsp. paracasei ATCC 25303 C2 24.1 L. casei subsp. rhamnosus 43 C3 25.1 L. casei 8 C3 25.1 L. casei 65 C3 25.1 L. plantarum ATCC 14917 T D 30.1 L. plantarum UH 2153 D 30.1 L. plantarum ATCC 10012 D 30.2 L. plantarum ATCC 11974 D1 31.1 L. plantarum ATCC 14431 D2 32.1 L. plantarum ATCC 8014 D3 33.1 L. plantarum 260 D3 33.1 L. plantarum RC-20 D4 34.1 L. acidophilus 75 D5 34.2 US2-A D6 35.1 US2-B D6 35.1 US2-C D6 35.1 US3-A D7 35.2 US3-B D7 35.2 US6-A D8 36.1 US6-B D8 36.1 US6-C D8 36.1 C4-A D8 36.1 C4-B D8 36.1 US8-A D9 37.1 L. fermentum ATCC 14931 T E 40.1 L. fermentum ATCC 23271 E1 40.2 L. fermentum ATCC 8289 E2 40.3 L. fermentum ATCC 9338 E2 40.3 L. fermentum ATCC 11739 E3 41.1 L. fermentum ATCC 14932 E4 42.1 L. fermentum B-54 E5 43.1 L. acidophilus RC-14 E5 43.1 L. acidophilus RC-14 (subcultured 20 times) E5 43.1 L. acidophilus ATCC 4356 T F 50.1 US4 F 50.2 L. acidophilus T-13 F1 51.1 L. jensenii ATCC 25258 T G 60.1 Open in a separate window Molecular typing of Lactobacillus isolates with respect to ATCC typed strains Nine health food products were examined.

Techniques: Southern Blot, Hybridization